What is HPV Hybrid Capture?

What is HPV Hybrid Capture?

NAME AND INTENDED USE. ] The HC2 High-Risk HPV DNA Test using Hybrid Capture®2 (HC2) technology is a nucleic acid hybridization assay with signal amplification using microplate chemiluminescence for the qualitative detection of thirteen high-risk types of human papillomavirus (HPV) DNA in cervical specimens.

What does Hybrid Capture mean?

(hī’brid kap’shŭr) A signal amplification method. An RNA probe is annealed to target DNA; then a captured antibody binds the DNA-RNA hybrid to a solid surface.

What is HPV DNA detected?

A laboratory test in which cells are scraped from the cervix to look for DNA of human papillomaviruses (HPV). HPV can cause abnormal tissue growth (for example, warts) and other changes to cells. Infection for a long time with certain types of HPV can cause cervical cancer.

What does HPV DNA high risk detected mean?

An abnormal result means you have a high-risk type of HPV. High-risk types of HPV may cause cervical cancer and cancer of the throat, tongue, anus, or vagina. Most of the time, cervical cancer related to HPV is due to the following types: HPV-16 (high risk type)

What is the principle of hybrid capture system?

Principle of Hybrid Capture (HC) HPV Detection Antibodies conjugated with alkaline phosphate and specific for DNA-RNA hybrids react with hybrids which are then subsequently detected using a chemiluminescent substrate. The signal amplification occurs as several conjugated antibodies bind to each hybrid.

How is cobas HPV test done?

The cobas® HPV Test is a qualitative in vitro test for the detection of Human Papillomavirus in cervical specimens collected by a clinician using an endocervical brush/spatula and placed in the ThinPrep Pap Test PreservCyt® Solution or using a cervical broom and placed in SurePath™ Preservative Fluid.

What is Aptima HPV assay?

The Aptima® HPV assay is the first FDA-approved test for HPV. mRNA, and the test detects mRNA from 14 high-risk HPV types. associated with cervical cancer.1 The Aptima HPV assay can. be used together with the Pap for women age 30 and older, as. well as for reflex on ASC-US Pap results.1.