What is immunoelectrophoresis IEP?

What is immunoelectrophoresis IEP?

Immunoelectrophoresis (IEP) was the first practical method that combined electrophoresis and -immunoprecipitation for identifying and characterizing proteins within complex mixtures.

What is immunoelectrophoresis blood test?

Definition. Serum immunoelectrophoresis is a lab test that measures proteins called immunoglobulins in the blood. Immunoglobulins are proteins that function as antibodies , which fight infection. There are many types of immunoglobulins that fight different types of infections.

Is immunofixation the same as immunoelectrophoresis?

Immunofixation identified the proteins unambiguously in all six sera examined, whereas immunoelectrophoresis gave ambiguous results in one of the sera with high protein concentration and in three of the sera with low protein concentration. The superiority of immunofixation resulted from its greater resolution.

What is immunoelectrophoresis IEP and how it is done?

Immunoelectrophoresis (IEP) is an older method for qualitative analysis of M-proteins in serum and urine. IEP is a two-step procedure that combines the principles of zone electrophoresis and immunodiffusion. The shape, position, and size of each arc demonstrate the specific characteristics of each protein.

What is the principle of immunoelectrophoresis?

Immunoelectrophoresis (IEP) encompasses two principles: firstly, an electrophoresis for separation of the protein antigen in semi-solid media, followed by an immunodiffusion against the antiserum resulting in precipitin formation (Csako, 2019).

Why is Immunofixation done?

The immunofixation blood test is used to identify proteins called immunoglobulins in blood. Too much of the same immunoglobulin is usually due to different types of blood cancer. Immunoglobulins are antibodies that help your body fight infection.

How Immunofixation is done?

Technique. The technique consists of depositing a serum (or urine which has been previously concentrated) sample on a gel. After application of an electric current that allows the separation of proteins according to their size, antibodies specific for each type of immunoglobulin are laid upon the gel.

For what purpose immunoelectrophoresis is used?

The immunoelectrophoresis-serum test (IEP-serum) is a blood test used to measure the types of Ig present in your blood, especially IgM, IgG, and IgA. The IEP-serum test is also known by the following names: immunoglobulin electrophoresis-serum test.

What are the types of immunoelectrophoresis?

Four types of immunoelectrophoresis (IEP) have been used: electroimmunoassay (EIA also called ‘rocket’ or ‘Laurell rocket’), classical IEP, immunofixation electrophoresis (IFE) and immunoprecipitation of proteins after capillary electrophoresis.

What does a positive immunofixation blood test mean?

Positive results from the test indicate the presence of abnormal Ig. This may suggest the existence of an underlying health condition, such as: an immune system disorder. multiple myeloma. Waldenstrom’s macroglobulinemia.

What does immunofixation test mean?

Definition. The immunofixation blood test is used to identify proteins called immunoglobulins in blood. Too much of the same immunoglobulin is usually due to different types of blood cancer. Immunoglobulins are antibodies that help your body fight infection.